МЕТОД ВЭЖХ ДЛЯ ОПРЕДЕЛЕНИЯ ОСТАТКОВ НЕКОТОРЫХ АНТИБИОТИКОВ В СТОЧНЫХ ВОДАХ РАЗЛИЧНЫХ БОЛЬНИЦ В ГОРОДЕ БАГДАД, ИРАК
Аннотация
Антибиотики представляют наибольшую угрозу для почвы и водных экосистем среди различных терапевтических групп лекарств (которые включают отпускаемые по рецепту лекарства и средства для лечения рака). Сильнейшие лекарства ‒ антибиотики ‒ использовались для остановки роста микроорганизмов или их уничтожения. С помощью технологии высокоэффективной жидкостной хроматографии с флуоресцентным детектированием были определены количества левофлоксацина и тетрациклина в сточных водах трех больниц (Медикал Сити, Аль-Кинди и Аль-Ярмук). В этом исследовании были выбраны левофлоксацин и тетрациклин, поскольку они являются наиболее важными загрязнителями воды. Эти остатки антибиотиков были разделены и измерены с использованием метода градиентного элюирования на колонке с обращенной фазой C18. Комбинация метанола и деионизированной воды составляла систему подвижной фазы. Для 20-минутного периода анализа длины волн возбуждения и излучения детектора были установлены на 310 и 420 нм соответственно. Очистку картриджа ТФЭ проводили после экстракции с использованием цитратного буфера с pH 4. Прекрасные линейные зависимости (R2 > 0,9998) наблюдались на калибровочных кривых для левофлоксацина и тетрациклина в концентрациях от 10 до 40 мкг/мл. Пределы обнаружения и количественного определения левофлоксацина и тетрациклина составили 0,61 мкг/мл, 2,04 мкг/мл, 0,46 мкг/мл и 1,54 мкг/мл соответственно. С использованием предложенной методики был успешно применен анализ остатков антибиотиков в различных пробах сточных вод. Результаты показывают наличие тетрациклина и левофлоксацина во всех пробах сточных вод. Однако в Аль-Кинди их концентрация была выше, чем в Медикал-Сити и Аль-Ярмуке. Предложенный метод может быть применен к ряду медицинских продуктов в различных источниках сточных вод, таких как больницы и промышленные предприятия.
Для цитирования:
Алаа Абдулвахид Джасим, Васан Абдулвахид Джасим, Джасим М.С. Джамур Метод ВЭЖХ для определения остатков некоторых антибиотиков в сточных водах различных больниц в городе Багдад, Ирак. Изв. вузов. Химия и хим. технология. 2024. Т. 67. Вып. 6. С. 21-28. DOI: 10.6060/ivkkt.20246706.7035.
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