MICROPARTICLES OF POLYELECTROLYTE COMPLEXES BASED ON POLY-N,N-DIALLYL-N,N-DIMETHYLAMMONIUM CHLORIDE AND OXIDIZED ARABINOGALACTAN MODIFIED WITH HISTIDINE
Abstract
Complexation of poly-N,N-diallyl-N,N-dimethylammonium chloride and carboxylated arabinogalactan with histidine has been studied by a complex of a number of physicochemical methods (UV-, IR-, 1H NMR-, 13C NMR-spectroscopy and pH-metry). When the functional groups of polymers interact with the amino acid, complexes of the composition 1:1 for arabinogalactan and 2:1 for poly-N,N-diallyl-N,N-dimethylammonium chloride are formed. The stability constants and thermodynamic characteristics of the complexation process have been calculated. It was shown by IR spectroscopy that the carboxyl groups of the polyanion interact with the amino groups and the nitrogen atom of the imidazole ring of the amino acid. The interaction with the polycation involves the carboxyl groups of histidine. The formation of microparticles of polyelectrolyte complexes based on poly-N,N-diallyl-N,N-dimethylammonium chloride and carboxylated arabinogalactan, as well as complexes based on polyelectrolytes modified by histidine, has been studied by turbidimetric titration and laser light scattering methods. The lyophilizing ability of polyelectrolytes and, accordingly, the size of microparticles depends on the number of ionogenic groups in polymers. It is shown that chemical modification of polyelectrolytes, leading to a decrease in the content of ionogenic groups in macromolecules, leads to an increase in the size of microparticles of polyelectrolyte complexes. The conditions for obtaining complex systems have been optimized from the point of view of aggregate stability and particle size. It was found that there is a linear relationship between the ratio of the complex components and the average particle size - the particle size increases with an increase in the molar ratio (p-anion / p-cation) of polyelectrolytes in the complex. It has been shown that precipitates of polyelectrolyte complexes isolated from aqueous dispersions are well preserved in the form of dry powders, and upon dispersion in water they give microparticles with sizes comparable to those of the initial complexes. Similarly, the problem of long-term storage of physiologically active microparticles can be solved.
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